Deteksi Virus Hepatitis A di Lingkungan
Abstract
Introduction. Hepatitis A virus (HAV) is a significant waterborne human pathogen. More than 140 viruses are known to be excreted in faeces by infected persons including HAV. Detection of virus is very important for management. The objective of study is to identify concentration methods for virus isolation from environment sample and type of PCR to detect HAV. Method: Literature review was conducted using electronic database: google schoolar with key words Detection of Hepatitis A in an environment, its journal published in 2000 – 2019. Only article in English included in study. Results: Environmental samples can be processed to detect HAV. Virus can be detected after water samples had been concentrated. The concentrate then tested with PCR (Qualitative,q uantitative real-time RT–PCR or Nested PCR) to detect the presence of HAV in environmental samples. Consclusion. The real-time RT–PCR technique is more efficient, faster, no need for post-PCR confirmation of amplified product, no correlation between viral contamination and bacterial indicators. The detection rate for nested RT–PCR was lower than quantitative real-time RT–PCR. Real-time PCR was more sensitive than nested RT-PCR and it could enumerate HAV RNA.
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