Reduksi Biomassa dan Viabilitas Biofilm Enterococcus faecalis melalui Paparan Medan Elektromagnetik

Shafa Noer, Ahmad Jahrudin, Zakiah Fithah ‘Aini

Abstract


Biofilm of Enterococcus faecalis is known for its high resistance to various antimicrobial agents and is a major contributor to the failure of chronic infection therapies, thus requiring alternative approaches to inhibit its formation. Electromagnetic fields (EMFs) have been reported to modulate microbial cellular activity, but the use of simple and controlled EMF circuits to suppress biofilm formation remains rarely investigated. This study aimed to evaluate the effect of an assembled EMF exposure on the biomass and viability of E. faecalis biofilms. Bacterial cultures were exposed to EMF under fixed parameters during incubation, while the control group received no treatment. Biofilm biomass was quantified using the Crystal Violet (CV) method, and cellular metabolic activity was analyzed using the MTT assay. The results showed that the control group exhibited higher CV and MTT absorbance values than the treatment group, indicating a reduction in biofilm formation and cell viability due to EMF exposure. These findings suggest that electromagnetic fields have potential as a non-chemical method for modulating E. faecalis biofilms.

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